CST, 74575SF, RGS14 (E4N2Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying RGS14 mouse. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, RGS14 (E4N2Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #74575) is ready to ship. Product Usage Information This product is the carrier free version of product #76703. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 76703 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity RGS14 (E4N2Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total RGS14 protein. Non-specific staining in fixed frozen mouse kidney may be observed by immunofluorescence. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse RGS14 protein. Background Regulator of G-protein signaling (RGS) proteins are a structurally diverse family of more than 30 member proteins enriched in brain that typically limit neurotransmitter receptor- and G protein-linked signaling in the CNS and elsewhere by serving as GTPase-activating proteins (GAPs) (1). RGS14 is a multifunctional signaling protein that regulates post-synaptic plasticity in neurons and acts as a scaffolding protein that integrates G protein, Ras/ERK, and calcium/calmodulin signaling pathways essential for spine plasticity and cell signaling (2). It is highly enriched within pyramidal cells of hippocampal area CA2, allowing RGS14 to naturally suppress long-term potentiation (LTP) and structural plasticity in that region (2-4). RGS14 has been shown to directly interact with two key Ca -activated proteins in CA2 neurons: calcium/calmodulin and CaMKII (5). Alternate Names rap1/rap2 interacting protein; RAP1/RAP2-interacting protein; Regulator of G-protein signaling 14; RGS14; RPIP1 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG