CST, 75000S, PGLYRP-1/PGRP-S (E5M8P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PGLYRP1 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PGLYRP-1/PGRP-S (E5M8P) Rabbit Monoclonal Antibody (CST #75000) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PGLYRP-1/PGRP-S (E5M8P) Rabbit Monoclonal Antibody recognizes endogenous levels of total PGLYRP-1/PGRP-S protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg181 of mouse PGLYRP-1/PGRP-S protein. Background The human peptidoglycan recognition protein (PGRP) family is composed of four independent genes named for the relative length of their translated product. Human PGRP family proteins are soluble pattern-recognition receptors of the innate immune response that bind peptidoglycan and Gram-positive bacteria, and have direct bactericidal activity (1,2). PGRP-short (PGRP-S), also known as PGLYRP-1 and Tag7, is 196 amino acids (aa) in length (3). PGLYRP-1/PGRP-S is a secreted glycoprotein expressed by neutrophils that participates in killing phagocytized bacteria (4,5). PGLYRP-1/PGRP-S also has been shown to form a stable cytotoxic complex with the heat shock protein Hsp70 to induce tumor cell death (6,7). PGRP-long (PGRP-L), also known as PGLYRP-2, is 576 aa long. PGLYRP-2/PGRP-L is an N-acetylmuramoyl-L-alanine amidase that hydrolyzes bacterial peptidoglycan (8,9). PGRP-intermediate alpha, also known as PGLYRP-3, and PGRP-intermediate beta, also known as PGLYRP-4, are 341 aa and 373 aa long, respectively. PGLYRP-3 and PGLYRP-4 are co-expressed in the same cells and are exclusively secreted as homo- or heterodimers with antibacterial function (10). Alternate Names Cytokine tag7; O88593; Peptidoglycan recognition protein 1; Peptidoglycan recognition protein short; Pglyrp; Pglyrp1; PGRP; PGRP-S; PGRP1; Pgrps; Tag; Tag7; Tas; Tasg7; Tnfs; Tnfsf3l; tumor necrosis factor super family 3-like Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG