CST, 84292T, DPEP-1/MDP (E5H7T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DPEP1 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, DPEP-1/MDP (E5H7T) Rabbit Monoclonal Antibody (CST #84292) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity DPEP-1/MDP (E5H7T) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse DPEP-1/MDP protein. This antibody does not cross-react with human DPEP-1/MDP protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys60 of mouse DPEP-1/MDP protein. Background Dipeptidase-1 (DPEP-1, RDP, MDP) is a GPI-linked glycoprotein expressed in the epithelial brush border of the kidney epithelium and exists as a disulfide-linked homodimer (1). Research studies have shown that the enzymatic activity of DPEP-1 is involved in kidney metabolism of glutathione, leukotrienes, and β-lactam antibiotics (2,3). Independent of its enzymatic activity, DPEP-1 has been implicated in pathologic inflammation by acting as an adhesion receptor for neutrophils on endothelial cells within the lung (4). DPEP-1 has also been implicated in human cancer and numerous studies have demonstrated that expression of this enzyme is upregulated in colorectal cancer and promotes metastasis (5-7). Alternate Names AI327012; Beta-lactamase; Dipeptidase 1; dipeptidase 1 (renal); DPEP-1; Dpep1; MBD; MBD-1; Mbd1; membrane bound dipeptidase type II; Membrane-bound dipeptidase 1; Microsomal dipeptidase; Rdp; Renal dipeptidase Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG