CST, 84651S, PD-1 (Intracellular Domain) (D7D5W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PDCD1 mouse. Validated for WB,IP,IHC,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, PD-1 (Intracellular Domain) (D7D5W) Rabbit Monoclonal Antibody (CST #84651) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 55789 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity PD-1 (Intracellular Domain) (D7D5W) Rabbit Monoclonal Antibody recognizes endogenous levels of total PD-1 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala242 of mouse PD-1 protein. Background The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T cells, PD-1 is expressed in activated B cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10). Alternate Names CD279; Ly101; mPD-1; PD-; PD-1; Pd1; Pdc; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; Programmed cell death protein 1; Protein PD-1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 40-75 Source/Isotype: Rabbit IgG