CST, 85270S, CCL3/MIP-1 alpha (F2M8Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CCL3 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CCL3/MIP-1 alpha (F2M8Q) Rabbit Monoclonal Antibody (CST #85270) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage For a carrier free (BSA and azide free) version of this product see product # 90323 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CCL3/MIP-1 alpha (F2M8Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total CCL3/MIP-1α protein. This antibody detects a 42 kDa band of unknown origin in some cell lines. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CCL3/MIP-1α protein. Background Chemokine (C-C motif) ligand 3 (CCL3), also known as macrophage inflammatory protein-1α (MIP-1α), is a 10 kDa member of the C-C or β family of chemokines that play key roles in immune surveillance, inflammation, and infection (1). CCL3 shares 68% amino acid identity with CCL4 (MIP-1β) and 94% amino acid identity with LD78β (1). CCL3 is produced by activated monocytes, activated macrophages, NK cells, and T cells (1). There are two receptors for CCL3: CCR1 and CCR5 (1). CCL3 inhibits R5 HIV-1 infection by competing with the virus for its co-receptor, CCR5 (1). CCL3 possesses hematopoietic stem cell inhibitory activity, suppressing their proliferation, but has been shown to promote proliferation of more mature progenitors (2-4). CCL3 has been implicated in several forms of leukemia, in which malignant cells may lose responsiveness to CCL3 or produce high levels of CCL3 (2). CCL3 polymorphisms are associated with risk for the development of rheumatoid arthritis and ulcerative colitis (5,6). CCL3 expression is upregulated in the brains of patients with Alzheimer's disease (AD) with dementia, and reduced expression of CCL3 is observed in AD patients resilient to dementia (7). Alternate Names AI323804; C-C motif chemokine 3; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; Heparin-binding chemotaxis protein; L2G25B; LD78alpha; Macrophage inflammatory protein 1-alpha; macrophage inflammatory protein-1alpha; MIP-1-alpha; MIP-1alpha; MIP1; MIP1 (a); MIP1-(a); MIP1-alpha; Mip1a; Scya3; SIS-alpha; Small-inducible cytokine A3; TY-5 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG