CST, 85472S, SEC24B (F6A7W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SEC24B mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SEC24B (F6A7W) Rabbit Monoclonal Antibody (CST #85472) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SEC24B (F6A7W) Rabbit Monoclonal Antibody recognizes endogenous levels of total SEC24B protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu292 of mouse SEC24B protein. Background Coat Protein Complex II (COPII) is composed of five cytosolic proteins: Sec23/24 complex, Sec13/31 complex, and Sar1. COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex, thereby forming a prebudding complex that directly binds target molecules (1-3). The prebudding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec24 subunit of COPII coat is thought to play a critical role in cargo selection (2,6). It binds directly to cargo proteins at the ER and brings them to COPII vesicles through interaction with Sec23. There are four Sec24 isoforms in human cells: Sec24A, Sec24B, Sec24C, and Sec24D (7). In mice, mutations in Sec24B have been linked to developmental defects (8,9). Sec24bB has also been identified as a novel regulator of ferroptosis in microglia which can contribute to neurodegeneration (10). Alternate Names AI605202; protein transport protein Sec24B; SEC2; SEC24; Sec24 related gene family, member B; Sec24 related gene family, member B (S. cerevisiae); Sec24-related gene family, member B (S. cerevisiae); Sec24b Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG