Proteintech, 10001-0-AP, 6*His, His-tag Polyclonal antibody

Size: 150ul The 6*His, His-tag (10001-0-AP) by Proteintech is a Polyclonal antibody targeting 6*His, His-tag in WB, ELISA applications with reactivity to recombinant protein samples 10001-0-AP targets 6*His, His-tag in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples. Tested Applications Positive WB detected in: recombinant protein Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein. Specification Tested Reactivity: recombinant protein Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: 6*His, His-tag Calculated Molecular Weight: 1 kDa RRID: AB_11232228 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: HISTAG Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.