Proteintech, 10023-1-AP, JNK Polyclonal antibody

Size: 20ul / 150ul The JNK (10023-1-AP) by Proteintech is a Polyclonal antibody targeting JNK in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 10023-1-AP targets JNK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: NIH/3T3 cells, A431 cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, RAW 264.7 cells, RAW264.7, SH-SY5Y cells Positive IHC detected in: human prostate cancer tissue, human lung cancer tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information FunctionJNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.Tissue specificityJNK1 is ubiquitously expressed.Involvement in diseaseJNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.IsoformsThere are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.Post-translational modificationsJNK1 is phosphorylated by MAPK kinases (MAPKK).Cellular localizationJNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0019 Product name: Recombinant human JNK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: JNK of NM_138982 Sequence: Predict reactive species Full Name: mitogen-activated protein kinase 8 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 40-45 kDa, 50-55 kDa GenBank Accession Number: NM_138982 Gene Symbol: JNK Gene ID (NCBI): 5599 RRID: AB_2281669 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P45983 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.