Proteintech, 10045-1-Ig, GRB7 Polyclonal antibody

Size: 20ul / 150ul The GRB7 (10045-1-Ig) by Proteintech is a Polyclonal antibody targeting GRB7 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 10045-1-Ig targets GRB7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A431 cells, HEK-293 cells Positive IP detected in: A431 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Grb7 belongs to a family of adapter proteins. This family, including Grb10, Grb7 and Grb 14, contains a praline-rich, a RA, a PH, a BPS and SH2 domain. Grb7 associate with a number of receptors including EGFR and FAK. Functions of Grb7 include mobility because it associates receptors such FAK, and direct evidence from overexpression of this proteins.This antibody recognize the N-terminal of GRB7. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0023 Product name: Recombinant human GRB7 protein Source: e coli. -derived, Unknow Tag: N/A Domain: Gst-KG-Grb7 of BC006535 Sequence: Predict reactive species Full Name: growth factor receptor-bound protein 7 Calculated Molecular Weight: 532 aa, 60 kDa Observed Molecular Weight: 60-70 kDa GenBank Accession Number: BC006535 Gene Symbol: GRB7 Gene ID (NCBI): 2886 RRID: AB_2232601 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q14451 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.