Proteintech, 10066-2-AP, HINFP Polyclonal antibody

Size: 20ul / 150ul The HINFP (10066-2-AP) by Proteintech is a Polyclonal antibody targeting HINFP in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 10066-2-AP targets HINFP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, human liver tissue, mouse liver tissue, rat liver tissue, L02 cells Positive IP detected in: L02 cells Positive IF/ICC detected in: Hela cells, L02 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0115 Product name: Recombinant human HINFP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 215-452 aa of BC001073 Sequence: LDHIRRQTSLDQQHFQCSHCSKRFATERLLRDHMRNHVNHYKCPLCDMTCPLPSSLRNHMRFRHSEDRPFKCDCCDYSCKNLIDLQKHLDTHSEEPAYRCDFENCTFSARSLCSIKSHYRKVHEGDSEPRYKCHVCDKCFTRGNNLTVHLRKKHQFKWPSGHPRFRYKEHEDGYMRLQLVRYESVELTQQLLRQPQEGSGLGTSLNESSLQGIILETVPGEPGRKEEEEEGKGSEGTA Predict reactive species Full Name: histone H4 transcription factor Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 60-70 kDa GenBank Accession Number: BC001073 Gene Symbol: HINFP Gene ID (NCBI): 25988 RRID: AB_2117412 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BQA5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.