Proteintech, 10157-1-AP, NPRL2 Polyclonal antibody

Size: 20ul / 150ul The NPRL2 (10157-1-AP) by Proteintech is a Polyclonal antibody targeting NPRL2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 10157-1-AP targets NPRL2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: rat pancreas tissue, HeLa cells, mouse pancreas tissue Positive IP detected in: mouse pancreas tissue Positive IHC detected in: human lung cancer tissue, human pancreas tissue, human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information NPRL2 (NPR2 Like, GATOR1 Complex Subunit) is a Protein Coding gene associated with Epilepsy, Familial Focal. As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. Homologous to yeast nitrogen permease (candidate tumor suppressor) (NM_006545, BC021984) gene encodes a protein of molecular mass of 50 kDa. Data by using overlapping and nested homozygous deletions methods indicate that this protein might be a tumor suppressor involved in the development of lung cancers. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0210 Product name: Recombinant human NPR2L,TUSC4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-270 aa of BC002791 Sequence: GCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAMEKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVSMEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKDKEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQYSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGH Predict reactive species Full Name: tumor suppressor candidate 4 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 40, 44-50 kDa GenBank Accession Number: BC002791 Gene Symbol: TUSC4 Gene ID (NCBI): 10641 RRID: AB_514025 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WTW4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.