Proteintech, 10193-2-AP, RCN2 Polyclonal antibody

Size: 20ul / 150ul The RCN2 (10193-2-AP) by Proteintech is a Polyclonal antibody targeting RCN2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 10193-2-AP targets RCN2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse lung tissue, human brain tissue, HeLa cells Positive IP detected in: mouse lung tissue Positive IHC detected in: human gliomas tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:350-1:1400 Background Information Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0247 Product name: Recombinant human RCN2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 24-209 aa of BC004892 Sequence: KAEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLE Predict reactive species Full Name: reticulocalbin 2, EF-hand calcium binding domain Calculated Molecular Weight: 37 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC004892 Gene Symbol: RCN2 Gene ID (NCBI): 5955 RRID: AB_2180018 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14257 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.