Proteintech, 10206-1-AP, NCK2 Polyclonal antibody

Size: 20ul / 150ul The NCK2 (10206-1-AP) by Proteintech is a Polyclonal antibody targeting NCK2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 10206-1-AP targets NCK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: U-937 cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Lots of the protein-protein interactions that are essential for eukaryotic intracellular signal transduction are mediated by protein binding modules including SH2, SH3, and LIM domains. NCK is a SH3- and SH2-containing adaptor protein implicated in coordinating various signaling pathways, including those of growth factor receptors and cell adhesion receptors. NCK-2 is a ubiquitously expressed and NCK-related adaptor protein comprising primarily three N-terminal SH3 domains and one C-terminal SH2 domain. NCK-2 interacts with PINCH, a LIM-only protein implicated in integrin-linked kinase signaling, and recognizes several key components of growth factor kinase-signaling pathways including EGF receptors, PDGF receptor, and IRS-1. NCK-2 may thus function as an adaptor protein connecting the growth factor receptor-signaling pathways with the integrin-signaling pathways. NCK-2 also interacts with focal adhesion kinase (FAK), a cytoplasmic protein tyrosine kinase critically involved in the cellular control of motility. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0262 Product name: Recombinant human NCK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 8-235 aa of BC000103 Sequence: IAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWW Predict reactive species Full Name: NCK adaptor protein 2 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 43 kDa GenBank Accession Number: BC000103 Gene Symbol: NCK2 Gene ID (NCBI): 8440 RRID: AB_2150571 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43639 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.