Proteintech, 10210-2-AP, RUVBL1 Polyclonal antibody

Size: 20ul / 150ul The RUVBL1 (10210-2-AP) by Proteintech is a Polyclonal antibody targeting RUVBL1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 10210-2-AP targets RUVBL1 in WB, IHC, IF/ICC, IP, CoIP, CHIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: DU 145 cells, HEK-293 cells, HeLa cells, K-562 cells, NIH/3T3 cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse liver tissue, human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0281 Product name: Recombinant human RUVBL1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC002993 Sequence: MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFD Predict reactive species Full Name: RuvB-like 1 (E. coli) Calculated Molecular Weight: 456 aa, 50 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC002993 Gene Symbol: RUVBL1 Gene ID (NCBI): 8607 RRID: AB_2184405 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y265 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.