Proteintech, 10228-1-AP, Nucleobindin 1 Polyclonal antibody

Size: 20ul / 150ul The Nucleobindin 1 (10228-1-AP) by Proteintech is a Polyclonal antibody targeting Nucleobindin 1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples 10228-1-AP targets Nucleobindin 1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: MCF7 cells, A431 cells, HeLa cells, HepG2 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Nucleobindin-1 (NUCB1), also known as Calnuc, is a highly conserved, multifunctional protein widely expressed in tissues and cells.It has been proposed to contain both calcium- and DNA-binding motifs, including two EF-hands, a basic amino-acid-rich region and a leucine zipper.Western blotting revealed a band at approximately 63 kDa, corresponding to the expected size of the NUCB1 protein(PMID: 15255074) Specification Tested Reactivity: human, mouse Cited Reactivity: mouse, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0290 Product name: Recombinant human NUCB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 127-286 aa of BC002356 Sequence: VQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDM Predict reactive species Full Name: nucleobindin 1 Calculated Molecular Weight: 55 kDa Observed Molecular Weight: 63 kDa GenBank Accession Number: BC002356 Gene Symbol: NUCB1 Gene ID (NCBI): 4924 RRID: AB_2153655 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q02818 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.