Proteintech, 10385-1-AP, RFC5 Polyclonal antibody

Size: 20ul / 150ul The RFC5 (10385-1-AP) by Proteintech is a Polyclonal antibody targeting RFC5 in IP, ELISA applications with reactivity to human, mouse samples 10385-1-AP targets RFC5 in WB, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IP detected in: HeLa cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C(RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. Replication factor C5 (RFC5, synonyms: RFC36, MGC1155) is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA and form a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0584 Product name: Recombinant human RFC5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 68-219 aa of BC001866 Sequence: STILACAKQLYKDKEFGSMVLELNASDDRGIDIIRGPILSFASTRTIFKKGFKLVILDEADAMTQDAQNALRRVIEKFTENTRFCLICNYLSKIIPALQSRCTRFRFGPLTPELMVPRLEHVVEEEKVDISEDGMKALVTLSSGDMRRALNI Predict reactive species Full Name: replication factor C (activator 1) 5, 36.5kDa Calculated Molecular Weight: 38 kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: BC001866 Gene Symbol: RFC5 Gene ID (NCBI): 5985 RRID: AB_2178603 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P40937 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.