Proteintech, 10425-1-AP, YY1AP1 Polyclonal antibody

Size: 20ul / 150ul The YY1AP1 (10425-1-AP) by Proteintech is a Polyclonal antibody targeting YY1AP1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 10425-1-AP targets YY1AP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: MDA-MB-231 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information YY1AP1 (YY1 associated protein 1), also known as HCCA2 (hepatocellular carcinoma-associated protein 2) or YY1AP, is a 796 amino acid protein that localizes to both the nucleus and the cytoplasm and exists as seven alternatively spliced isoforms. We detected 75 kDa and 50 kDa two isoforms. YY1AP1 is expressed ubiquitously with notable expression in lung, brain, spleen, pancreas, small intestine, heart, colon and skeletal muscle, YY1AP1 interacts with and enhances the transcriptional activation activity of YY1. YY1AP1 is expressed at high levels in liver carcinomas, suggesting a role in tumor transformation and metastasis. YY1AP1 may play a role in cell cycle regulation. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0692 Product name: Recombinant human YY1AP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC003500 Sequence: MKKPSAKQQKEVEKVKPQCKEVHQTLILDPAQRKRLQQQMQQHVQLLTQIHLLATCNPNLNPEASSTRICLKELGTFAQSSIALHHQYNPKFQTLFQPCNLMGAMQLIEDFSTHVSIDCSPHKTVKKTANEFPCLPKQVAWILATSKVFMYPELLPVCSLKAKNPQDKILFTKAEDNLLALGLKHFEGTEFLNPLISKYLLTCKTARQLTVRIKNLNMNRAPDNIIKFYKKTKQLPVLGKCCEEIQPHQWKPPIEREEHRLPFWLKASLPSIQEELRHMADGAREVGNMTGTTEINSDQGLEKDNSELGSETRYPLLLPKGVVLKLKPVADRFPKKAWRQKRSSVLKPLLIQPSPSLQPSFNPGKTPAQSTHSEAPPSKMVLRIPHPIQPATVLQTVPGV Predict reactive species Full Name: YY1 associated protein 1 Calculated Molecular Weight: 75 kDa Observed Molecular Weight: 75 kDa and 50 kDa GenBank Accession Number: BC003500 Gene Symbol: YY1AP1 Gene ID (NCBI): 55249 RRID: AB_2218515 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H869 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.