Proteintech, 10499-1-AP, TFII I Polyclonal antibody

Size: 20ul / 150ul The TFII I (10499-1-AP) by Proteintech is a Polyclonal antibody targeting TFII I in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 10499-1-AP targets TFII I in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells Positive IHC detected in: human thyroid cancer tissue, human colon cancer tissue, human urothelial carcinoma tissue, mouse small intestine tissue, rat kidney tissue, rat small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information The GTF2I gene encodes a transcription factor. It negatively regulates agonist-induced calcium entry into cells by interfering with expression of the cation channel TRPC3 at the plasma membrane [PMID: 17023658]. As a transcription factor and/or as a regulator of intracellular calcium levels, GTF2I may play a role in the molecular basis of anxiety[PMID: 22578324]. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation [PMID: 16738337]. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0773 Product name: Recombinant human GTF2I protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-274 aa of BC004472 Sequence: MAQVAMSTLPVEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRKDFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEKMLRDQSAVVVQGLPEGVAFKHPENYDLATLKWILENKAGISFIIKRPFLEPKKHVGGRVMVTDADRSILSPGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGSHHSSEGNEGTEMEVPAEG Predict reactive species Full Name: general transcription factor II, i Calculated Molecular Weight: 112 kDa Observed Molecular Weight: 135 kDa GenBank Accession Number: BC004472 Gene Symbol: TFII I Gene ID (NCBI): 2969 RRID: AB_2114813 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P78347 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.