Proteintech, 10553-1-AP, PCSK2 Polyclonal antibody

Size: 20ul / 150ul The PCSK2 (10553-1-AP) by Proteintech is a Polyclonal antibody targeting PCSK2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 10553-1-AP targets PCSK2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IHC detected in: mouse brain tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PCSK2(Prohormone convertase 2) is also named as NEC2(neuroendocrine convertase 2), PC2 and belongs to the peptidase S8 family. It is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK2 is responsible for the release of glucagon from proglucagon in pancreatic A cells. This protein has 3 isoforms produced by alternative splicing. PCSK2 is biosynthesized as a 74-kDa inactive precursor which is matured to a 64-kDa active enzyme by autocatalytic removal of its prodomain (PMID: 8397508, 21092739) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0852 Product name: Recombinant human PCSK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 338-638 aa of BC005815 Sequence: GRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLGLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQDPEKIPSTGKLVLTLTTDACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGVLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKEELEEELDEAVERSLKSILNKN Predict reactive species Full Name: proprotein convertase subtilisin/kexin type 2 Calculated Molecular Weight: 70.5 kDa Observed Molecular Weight: 65-75 kDa GenBank Accession Number: BC005815 Gene Symbol: PCSK2 Gene ID (NCBI): 5126 RRID: AB_2299124 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16519 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.