Proteintech, 10574-1-AP, SUV39H1 Polyclonal antibody

Size: 20ul / 150ul The SUV39H1 (10574-1-AP) by Proteintech is a Polyclonal antibody targeting SUV39H1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 10574-1-AP targets SUV39H1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HCT 116 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse small intestine tissue, C6 cells, NIH/3T3 cells Positive IP detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Histone-lysine N-methyltransferase (SUV39H1), a nuclear protein, moves to the centromeres during mitosis and specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. It plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. SUV39H1 has 2 isoforms with molecular weights of 48 and 49 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0854 Product name: Recombinant human SUV39H1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 7-277 aa of BC006238 Sequence: GCSVCCKSSWNQLQDLCRLAKLSCPALGISKRNLYDFEVEYLCDYKKIREQEYYLVKWRGYPDSESTWEPRQNLKCVRILKQFHKDLERELLRRHHRSKTPRHLDPSLANYLVQKAKQRRALRRWEQELNAKRSHLGRITVENEVDLDGPPRAFVYINEYRVGEGITLNQVAVGCECQDCLWAPTGGCCPGASLHKFAYNDQGQVRLRAGLPIYECNSRCRCGYDCPNRVVQKGIRYDLCIFRTDDGRGWGVRTLEKIRKNSFVMEYVGEI Predict reactive species Full Name: suppressor of variegation 3-9 homolog 1 (Drosophila) Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 48-50 kDa GenBank Accession Number: BC006238 Gene Symbol: SUV39H1 Gene ID (NCBI): 6839 RRID: AB_2196710 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43463 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.