Proteintech, 10713-1-AP, TPI1 Polyclonal antibody

Size: 20ul / 150ul The TPI1 (10713-1-AP) by Proteintech is a Polyclonal antibody targeting TPI1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 10713-1-AP targets TPI1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, A431 cells, HepG2 cells, rat liver tissue, rat kidney tissue, RAW 264.7 cells, mouse liver tissue, mouse kidney tissue, NIH/3T3 cells Positive IP detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, chicken, yeast, drosophila Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1175 Product name: Recombinant human TPI1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-249 aa of BC007086 Sequence: MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ Predict reactive species Full Name: triosephosphate isomerase 1 Calculated Molecular Weight: 27 kDa Observed Molecular Weight: 27 kDa GenBank Accession Number: BC007086 Gene Symbol: TPI1 Gene ID (NCBI): 7167 RRID: AB_2207716 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P60174 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.