Proteintech, 10739-1-AP, MKP-2/DUSP4 Polyclonal antibody

Size: 20ul / 150ul The MKP-2/DUSP4 (10739-1-AP) by Proteintech is a Polyclonal antibody targeting MKP-2/DUSP4 in WB, IP, ELISA applications with reactivity to human, mouse samples 10739-1-AP targets MKP-2/DUSP4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HCT 116 cells, SK-BR-3 cells Positive IP detected in: MDA-MB-231 cells Positive FC (Intra) detected in: MDA-MB-231 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1206 Product name: Recombinant human MKP-2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-394 aa of BC014565 Sequence: MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDCRPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPLRERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC Predict reactive species Full Name: dual specificity phosphatase 4 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 40-43 kDa GenBank Accession Number: BC014565 Gene Symbol: DUSP4 Gene ID (NCBI): 1846 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13115 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.