Proteintech, 10827-1-AP, TIP60/KAT5 Polyclonal antibody

Size: 20ul / 150ul The TIP60/KAT5 (10827-1-AP) by Proteintech is a Polyclonal antibody targeting TIP60/KAT5 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 10827-1-AP targets TIP60/KAT5 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-3 cells, rat liver tissue, HepG2 cells, Jurkat cells, HeLa cells, mouse liver tissue Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis. KAT5 exists some isoforms with MV 53-60 kDa Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1261 Product name: Recombinant human KAT5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 101-457 aa of BC000166 Sequence: ATPVPSETAPASVFPQNGAARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW Predict reactive species Full Name: K(lysine) acetyltransferase 5 Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 58 kDa, 70 kDa GenBank Accession Number: BC000166 Gene Symbol: KAT5 Gene ID (NCBI): 10524 RRID: AB_2128431 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92993 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.