Proteintech, 10828-1-AP, c-MYC Polyclonal antibody

Size: 20ul / 150ul The c-MYC (10828-1-AP) by Proteintech is a Polyclonal antibody targeting c-MYC in WB, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human samples 10828-1-AP targets c-MYC in WB, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA, EMSA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HT-29 cells, Jurkat cells, HeLa cells, A549 cells, HL-60 cells, human placenta tissue, Raji cells, SH-SY5Y cells, HepG2 cells, BxPC-3 cells, SW 1990 cells, Jurkat cells and Raji cells. Positive IP detected in: MCF-7 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells, HEK-293 cells, Saos-2 cells Positive FC (Intra) detected in: HeLa cells, NCCIT cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Functionc-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.Tissue specificityc-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.Involvement in disease·Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.·Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.IsoformsThere are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC (PMID: 12189186).Post-translational modificationsc-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.Cellular localizationc-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, pig, chicken, sheep, silkworm, p. lilacinum Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1263 Product name: Recombinant human MYC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 16-454 aa of BC000141 Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA Predict reactive species Full Name: v-myc myelocytomatosis viral oncogene homolog (avian) Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 62-65 kDa, 50 kDa GenBank Accession Number: BC000141 Gene Symbol: MYC Gene ID (NCBI): 4609 RRID: AB_2148585 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P01106 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.