Proteintech, 10838-1-AP, DNAJB2 Polyclonal antibody

Size: 20ul / 150ul The DNAJB2 (10838-1-AP) by Proteintech is a Polyclonal antibody targeting DNAJB2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 10838-1-AP targets DNAJB2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, A431 cells, mouse skeletal muscle tissue, rat heart tissue, mouse brain tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information The DNAJB2 (also known as HSJ1a, Heat-Shock Protein J1a) is a member of the DNAJ family of molecular chaperones which play important cellular roles in correct protein folding, in the response to protein misfolding, and in the degradation of misfolded proteins. It is expressed principally in the nervous system and is a key player in neuronal proteostasis maintenance. DNAJB2 has been proposed as a potent anti-aggregation chaperone for TDP-43, may play a role in the pathogenesis of ALS. DNAJB2 gene encodes two alternatively-spliced isoforms that differ in their C-terminus. The variant 1 form (V1 or HSJ1a) is expressed in both cytoplasm and nucleus, whereas the variant 2 form (V2 or HSJ1b) undergoes post-translational geranylgeranylation modification which mediates its attachment to the cytoplasmic side of the endoplasmic reticulum membrane. This antibody detects both isoforms of DNAJB2 with MW between 32-40 kDa. (PMID: 20395441) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1271 Product name: Recombinant human DNAJB2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-324 aa of BC011609 Sequence: MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEKKFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLELSRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPAGGREAQHRRQGRPKAQHQDPGLGGTQEGARGEATKRSPSPEEKASRCLIL Predict reactive species Full Name: DnaJ (Hsp40) homolog, subfamily B, member 2 Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 36-40 kDa GenBank Accession Number: BC011609 Gene Symbol: DNAJB2 Gene ID (NCBI): 3300 RRID: AB_2277491 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P25686 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.