Proteintech, 10875-1-AP, HSP47/SERPINH1 Polyclonal antibody

Size: 20ul / 150ul The HSP47/SERPINH1 (10875-1-AP) by Proteintech is a Polyclonal antibody targeting HSP47/SERPINH1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 10875-1-AP targets HSP47/SERPINH1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-1080 cells, A2780 cells, A431 cells, HeLa cells, NIH/3T3 cells, BALB/3T3 clone A31 cells Positive IHC detected in: human stomach cancer tissue, human colon tissue, human hepatocirrhosis tissue, human lung cancer tissue, mouse kidney tissue, mouse lung tissue, rat kidney tissue, rat lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. The Serpin Family H Member 1 (SERPINH1) gene encodes a protein called HSP47, which is required for the correct folding and secretion of collagen (PMID: 30128672). SERPINH1 has been implicated in several collagen-related diseases, such as, brittle bone disease, keloid scars, and lung fibrosis (PMID: 32091407). SERPINH1 also correlates with the grade of glioma, and regulates proliferation, migration and invasion of glioma cell lines (PMID: 24595468). SERPINH1 regulates the Wnt/β-catenin signaling pathway and modulates gastric cancer (GC) progression (PMID: 32091407). SERPINH1 is aberrantly expressed in cervical cancer, breast cancer, glioblastoma and colorectal cancer (PMID: 32091407). SERPINH1 promotes invasion and metastasis of breast cancer cells by regulating the expression of several extracellular matrix (ECM) proteins (PMID: 25744716). Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1301 Product name: Recombinant human SERPINH1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-418 aa of BC014623 Sequence: MRSLLLLSAFCLLEAALAAEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL Predict reactive species Full Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC014623 Gene Symbol: HSP47/SERPINH1 Gene ID (NCBI): 871 RRID: AB_2301500 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P50454 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.