Proteintech, 10992-1-AP, CSNK2A1 Polyclonal antibody

Size: 20ul / 150ul The CSNK2A1 (10992-1-AP) by Proteintech is a Polyclonal antibody targeting CSNK2A1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 10992-1-AP targets CSNK2A1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, PC-3 cells, K-562 cells, NIH/3T3 cells, Raji cells Positive IP detected in: HeLa cells Positive IHC detected in: human breast cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1452 Product name: Recombinant human CSNK2A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-391 aa of BC011668 Sequence: MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ Predict reactive species Full Name: casein kinase 2, alpha 1 polypeptide Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: BC011668 Gene Symbol: CSNK2A1 Gene ID (NCBI): 1457 RRID: AB_2245531 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P68400 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.