Proteintech, 11019-2-AP, SGTA Polyclonal antibody

Size: 20ul / 150ul The SGTA (11019-2-AP) by Proteintech is a Polyclonal antibody targeting SGTA in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 11019-2-AP targets SGTA in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, HeLa cells, human skeletal muscle tissue, mouse liver tissue, SH-SY5Y cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse brain tissue, human liver cancer tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1475 Product name: Recombinant human SGTA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC008885 Sequence: MDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKEMPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIELNPANAVYFCNRAAAYSKLGNYAGAVQDCERAICIDPAYSKAYGRMGLALSSLNKHVEAVAYYKKALELDPDNETYKSNLKIAELKLREAPSPTGGVGSFDIAGLLNNPGFMSMASNLMNNPQIQQLMSGMISGGNNPLGTPGTSPSQNDLASLIQAGQQFAQQMQQQNPELIEQLRSQIRSRTPSASNDDQQE Predict reactive species Full Name: small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 34-37 kDa GenBank Accession Number: BC008885 Gene Symbol: SGTA Gene ID (NCBI): 6449 RRID: AB_2188830 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43765 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.