Proteintech, 11029-1-AP, PSMB4 Polyclonal antibody

Size: 20ul / 150ul The PSMB4 (11029-1-AP) by Proteintech is a Polyclonal antibody targeting PSMB4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 11029-1-AP targets PSMB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse skeletal muscle tissue, human placenta tissue, human skeletal muscle tissue, MCF-7 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information PSMB4(Proteasome subunit beta type-4) is also named as PROS26 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The human protein PSMB4 is known mainly as an interaction partner for several proteins, suggesting an important function of binding not only to the lid, but also to the 20 S core of the proteasome. PSMB4 might be a major site for proteasome regulation, where signals from the outside might be transduced to the protease activities inside(PMID:15660529 ). The full length 30 kDa protein has a propeptide of 45 amino acid. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, pig, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1500 Product name: Recombinant human PSMB4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-264 aa of BC010088 Sequence: MEAFLGSRSGLWAGGPAPGQFYRIPSTPDSFMDPASALYRGPITRTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRVLYYRDARSYNRFQTATVTEKGVEIEGPLSTETNWDIAHMISGFE Predict reactive species Full Name: proteasome (prosome, macropain) subunit, beta type, 4 Calculated Molecular Weight: 29 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC010088 Gene Symbol: PSMB4 Gene ID (NCBI): 5692 RRID: AB_2172040 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P28070 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.