Proteintech, 11051-1-AP, GSNOR,ADH5 Polyclonal antibody

Size: 20ul / 150ul The GSNOR,ADH5 (11051-1-AP) by Proteintech is a Polyclonal antibody targeting GSNOR,ADH5 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 11051-1-AP targets GSNOR,ADH5 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, rat liver tissue, mouse kidney tissue Positive IP detected in: mouse liver tissue Positive IHC detected in: human prostate cancer tissue, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1533 Product name: Recombinant human GSNOR,ADH5 protein Source: e coli. -derived, Unknow Tag: N/A Domain: C16020 of BC070491 Sequence: Predict reactive species Full Name: alcohol dehydrogenase 5 (class III), chi polypeptide Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC070491 Gene Symbol: ADH5 Gene ID (NCBI): 128 RRID: AB_593422 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P11766 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.