Proteintech, 11057-1-AP, Cirhin Polyclonal antibody

Size: 20ul / 150ul The Cirhin (11057-1-AP) by Proteintech is a Polyclonal antibody targeting Cirhin in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 11057-1-AP targets Cirhin in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Neuro-2a cells Positive IP detected in: HepG2 cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Cirhin is a protein that in humans is encoded by the CIRH1A gene. Mutation of this gene has been associated with North American Indian childhood cirrhosis, a severe intrahepatic cholestasis that results in transient neonatal jaundice, and progresses to periportal fibrosis and cirrhosis in childhood and adolescence (PMID: 12417987). Catalog#11057-1-AP is a rabbit polyclonal antibody raised against the full-length of human CIRHIN. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1494 Product name: Recombinant human CIRH1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 335-686 aa of BC009348 Sequence: RCLISCSKKRQLLLFQFAHHLELWRLGSTVATGKNGDTLPLSKNADHLLHLKTKGPENIICSCISPCGSWIAYSTVSRFFLYRLNYEHDNISLKRVSKMPAFLRSALQILFSEDSTKLFVASNQGALHIVQLSGGSFKHLHAFQPQSGTVEAMCLLAVSPDGNWLAASGTSAGVHVYNVKQLKLHCTVPAYNFPVTAMAIAPNTNNLVIAHSDQQVFEYSIPDKQYTDWSRTVQKQGFHHLWLQRDTPITHISFHPKRPMHILLHDAYMFCIIDKSLPLPNDKTLLYNPFPPTNESDVIRRRTAHAFKISKIYKPLLFMDLLDERTLVAVERPLDDIIAQLPPPIKKKKFGT Predict reactive species Full Name: cirrhosis, autosomal recessive 1A (cirhin) Calculated Molecular Weight: 77 kDa Observed Molecular Weight: 70-77 kDa GenBank Accession Number: BC009348 Gene Symbol: Cirhin Gene ID (NCBI): 84916 RRID: AB_2877743 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q969X6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.