Proteintech, 11132-1-AP, Dysbindin Polyclonal antibody

Size: 20ul / 150ul The Dysbindin (11132-1-AP) by Proteintech is a Polyclonal antibody targeting Dysbindin in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 11132-1-AP targets Dysbindin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, human testis tissue, mouse testis tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Dysbindin, encoded by DTNBP1 (dystrobrevin binding protein 1), is a synaptic protein which is expressed in diverse neuronal populations throughout the brain. Genetic variation in DTNBP1 is associated with schizophrenia. Reduced expression of dysbindin has been reported in the dorsolateral prefrontal cortex (DLPFC) of schizophrenia cases. Several isoforms of dysbindin exist: 48-50 kDa of dysbindin-1A, 35-38 kDa of dysbindin-1B, and 30-32 kDa of dysbindin-1C. This antibody recognizes all of isoforms of dysbindin. (19617633) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1613 Product name: Recombinant human DTNBP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC011912 Sequence: MLETLRERLLSVQQDFTSGLKTLSDKSREAKVKSKPRTVPFLPKYSAGLELLSRYEDTWAALHRRAKDCASAGELVDSEVVMLSAHWEKKKTSLVELQEQLQQLPALIADLESMTANLTHLEASFEEVENNLLHLEDLCGQCELERCKHMQSQQLENYKKNKRKELETFKAELDAEHAQKVLEMEHTQQMKLKERQKFFEEAFQQDMEQYLSTGYLQIAERREPIGSMSSMEVNVDMLEQMDLMDISDQEALDVFLNSGGEENTVLSPALGPESSTCQNEITLQVPNPSELRAKPPSSSSTCTDSATRDISEGGESPVVQSDEEEVQVDTALATSHTDREATPDGGEDSDS Predict reactive species Full Name: dystrobrevin binding protein 1 Calculated Molecular Weight: 351 aa, 39 kDa Observed Molecular Weight: 48 kDa, 36 kDa GenBank Accession Number: BC011912 Gene Symbol: Dysbindin Gene ID (NCBI): 84062 RRID: AB_11182503 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96EV8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.