Proteintech, 11177-1-AP, OGFR Polyclonal antibody

Size: 20ul / 150ul The OGFR (11177-1-AP) by Proteintech is a Polyclonal antibody targeting OGFR in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 11177-1-AP targets OGFR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: COLO 320 cells, A549 cells, HEK-293 cells, L02 cells Positive IP detected in: COLO 320 cells Positive IHC detected in: human colon cancerNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: COLO 320 cells, L02 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1671 Product name: Recombinant human OGFR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 358-658 aa of BC014137 Sequence: LERSQGDEAGGHGEDRPEPLSPKESKKRKLELSRREQPPTEPGPQSASEVEKIALNLEGCALSQGSLRTGTQEVGGQDPGEAVQPCRQPLGARVADKVRKRRKVDEGAGDSAAVASGGAQTLALAGSPAPSGHPKAGHSENGVEEDTEGRTGPKEGTPGSPSETPGPSPAGPAGDEPAESPSETPGPRPAGPAGDEPAESPSETPGPSPAGPTRDEPAESPSETPGPRPAGPAGDEPAESPSETPGPRPAGPAGDEPAESPSETPGPSPAGPTRDEPAKAGEAAELQDAEVESSAKSGKP Predict reactive species Full Name: opioid growth factor receptor Calculated Molecular Weight: 73 kDa Observed Molecular Weight: 90-110 kDa GenBank Accession Number: BC014137 Gene Symbol: OGFR Gene ID (NCBI): 11054 RRID: AB_2156772 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NZT2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.