Proteintech, 11199-1-AP, ORM1/2 Polyclonal antibody

Size: 20ul / 150ul The ORM1/2 (11199-1-AP) by Proteintech is a Polyclonal antibody targeting ORM1/2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 11199-1-AP targets ORM1/2 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human plasma, L02 cells Positive IP detected in: L02 cells Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can recognize both ORM1 and ORM2. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1667 Product name: Recombinant human ORM2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC015964 Sequence: MALSWVLTVLSLLPLLEAQIPLCANLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQNQCFYNSSYLNVQRENGTVSRYEGGREHVAHLLFLRDTKTLMFGSYLDDEKNWGLSFYADKPETTKEQLGEFYEALDCLCIPRSDVMYTDWKKDKCEPLEKQHEKERKQEEGES Predict reactive species Full Name: orosomucoid 2 Calculated Molecular Weight: 24 kDa Observed Molecular Weight: 24-28 kDa, 41-47 kDa GenBank Accession Number: BC015964 Gene Symbol: ORM2 Gene ID (NCBI): 5005 RRID: AB_2158180 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P19652 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.