Proteintech, 11253-1-AP, SH3BGRL Polyclonal antibody

Size: 20ul / 150ul The SH3BGRL (11253-1-AP) by Proteintech is a Polyclonal antibody targeting SH3BGRL in WB, IHC, ELISA applications with reactivity to human, mouse samples 11253-1-AP targets SH3BGRL in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse colon tissue, mouse uterus tissue Positive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SH3BGRL, an adaptor protein, is one member of the SH3BGR family. SH3BGRL is ubiquitously expressed in varied human tissues and organs, including the bone marrow, heart, lung, liver, and kidney. SH3BGRL encodes a small protein with a conserved proline-rich PLPPQIF region and two motifs, Homer EVH1-binding and SH3-binding motifs. As a scaffold protein, SH3BGRL could participate in the protein-protein interaction for the integration or/and crosstalk of signal transduction, membrane trafficking, cytoskeletal rearrangements, and other key cellular processes. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1780 Product name: Recombinant human SH3BGRL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-114 aa of BC016709 Sequence: MVIRVYIASSSGSTAIKKKQQDVLGFLEANKIGFEEKDIAANEENRKWMRENVPENSRPATGYPLPPQIFNESQYRGDYDAFFEARENNAVYAFLGLTAPPGSKEAEVQAKQQA Predict reactive species Full Name: SH3 domain binding glutamic acid-rich protein like Calculated Molecular Weight: 13 kDa Observed Molecular Weight: 13 kDa GenBank Accession Number: BC016709 Gene Symbol: SH3BGRL Gene ID (NCBI): 6451 RRID: AB_3085369 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75368 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.