Proteintech, 11270-1-AP, MBD4 Polyclonal antibody

Size: 20ul / 150ul The MBD4 (11270-1-AP) by Proteintech is a Polyclonal antibody targeting MBD4 in WB, ELISA applications with reactivity to human samples 11270-1-AP targets MBD4 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human skin tissue, A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information MBD4, also named as MED1, is a 580 amino acid protein, which Interacts with MLH1. MBD4 as a mismatch-specific DNA N-glycosylase is involved in DNA repair and has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. MBD4 can also remove uracil or 5-fluorouracil in G:U mismatches. MBD4 exists some isoforms with MV 66 kDa and 30 kDa (The identification of a novel alternatively spliced form of the MBD4 DNA glycosylase). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1822 Product name: Recombinant human MBD4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 228-574 aa of BC011752 Sequence: VRKPKGKVTILKGIPIKKTKKGCRKSCSGFVQSDSKRESVCNKADAESEPVAQKSQLDRTVCISDAGACGETLSVTSEENSLVKKKERSLSSGSNFCSEQKTSGIINKFCSAKDSEHNEKYEDTFLESEEIGTKVEVVERKEHLHTDILKRGSEMDNNCSPTRKDFTEDTIPRTQIERRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWKFLEKYPSAEVARTADWRDVSELLKPLGLYDLRAKTIVKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQVHPEDHKLNKYHDWLWENHEKLSLS Predict reactive species Full Name: methyl-CpG binding domain protein 4 Calculated Molecular Weight: 66 kDa Observed Molecular Weight: 60-70 kDa GenBank Accession Number: BC011752 Gene Symbol: MBD4 Gene ID (NCBI): 8930 RRID: AB_2139871 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95243 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.