Proteintech, 11308-1-AP, GOLGA2/GM130 Polyclonal antibody

Size: 20ul / 150ul The GOLGA2/GM130 (11308-1-AP) by Proteintech is a Polyclonal antibody targeting GOLGA2/GM130 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, canine samples 11308-1-AP targets GOLGA2/GM130 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, canine samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells, human spleen tissue, MCF-7 cells, Calu-1 cells Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, MDCK cells, HEK-293 cells, HeLa cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635) Specification Tested Reactivity: human, canine Cited Reactivity: human, monkey, zebrafish, hamster, sheep Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1848 Product name: Recombinant human GM130;GOLGA2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 361-678 aa of BC014188 Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI Predict reactive species Full Name: golgi autoantigen, golgin subfamily a, 2 Calculated Molecular Weight: 111 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: BC014188 Gene Symbol: GM130 Gene ID (NCBI): 2801 RRID: AB_2115327 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q08379 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.