Proteintech, 11361-1-AP, Dynamitin (1-401 AA) Polyclonal antibody

Size: 20ul / 150ul The Dynamitin (1-401 AA) (11361-1-AP) by Proteintech is a Polyclonal antibody targeting Dynamitin (1-401 AA) in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 11361-1-AP targets Dynamitin (1-401 AA) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A375 cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, mouse testis tissue, rat brain tissue Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83aa is responsible for calmodulin binding. This antibody is against the full length p50 dynamitin. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1902 Product name: Recombinant human dynactin-2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-401 aa of BC014083 Sequence: MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK Predict reactive species Full Name: dynactin 2 (p50) Calculated Molecular Weight: 401 aa, 44 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC014083 Gene Symbol: Dynamitin Gene ID (NCBI): 10540 RRID: AB_2090609 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13561 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.