Proteintech, 11395-1-AP, GKAP1 Polyclonal antibody

Size: 20ul / 150ul The GKAP1 (11395-1-AP) by Proteintech is a Polyclonal antibody targeting GKAP1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 11395-1-AP targets GKAP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse testis tissue Positive IHC detected in: human cervical cancer tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information GKAP1, or GKAP42, is a novel male germ cell-specific 42-kDa protein isolated by Yuasa K et al. GKAP42 mRNA is specifically expressed in testis, where it is restricted to the spermatocytes and early round spermatids. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1914 Product name: Recombinant human GKAP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC014476 Sequence: MASAVLSSVPTTASRFALLQVDSGSGSDSEPGKGKGRNTGKSQTLGSKSTTNEKKREKRRKKKEQQQSEANELRNLAFKKIPQKSSHAVCNAQHDLPLSNPVQKDSREENWQEWRQRDEQLTSEMFEADLEKALLLSKLEYEEHKKEYEDAENTSTQSKVMNKKDKRKNHQGKDRPLTVSLKDFHSEDHISKKTEELSSSQTLSHDGGFFNRLEDDVHKILIREKRREQLTEYNGTDNCTAHEHNQEVVLKDGRIERLKLELERKDAEIQKLKNVITQWEAKYKEVKARNAQLLKMLQEGEMKDKAEILLQVDESQSIKNELTIQVTSLHAALEQERSKVKVLQAELAKYQGGRKGKRNSESDQCR Predict reactive species Full Name: G kinase anchoring protein 1 Calculated Molecular Weight: 366 aa, 42 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC014476 Gene Symbol: GKAP1 Gene ID (NCBI): 80318 RRID: AB_2877761 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5VSY0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.