Proteintech, 11428-1-AP, SerpinB3/SerpinB4 Polyclonal antibody

Size: 20ul / 150ul The SerpinB3/SerpinB4 (11428-1-AP) by Proteintech is a Polyclonal antibody targeting SerpinB3/SerpinB4 in WB, ELISA applications with reactivity to human samples 11428-1-AP targets SerpinB3/SerpinB4 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, COLO 320 cells, human saliva Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Squamous cell carcinoma antigens 1 and 2 (SCCA1 and 2, SERPIN B3 and B4) are members of the ovalbumin family of serine proteinase inhibitors. SCCA1 and SCCA2 are highly homologous proteins, 91% identical at the amino acid level, and probably evolving from a common ancestor gene. The neutral form of SCCA (SCCA1, or SERPINB3) is detected in the cytoplasm of normal and some malignant squamous cells, whereas the acidic form (SCCA2, or SERPINB4) is expressed primarily in malignant cells and is the major form found in the plasma of cancer patients. SCCA2 (SERPINB4), along with SCCA1(SERPINB3), can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation. This antibody can recognize both SerpinB3 and SerpinB4. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1964 Product name: Recombinant human SERPINB4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-390 aa of BC017401 Sequence: MNSLSEANTKFMFDLFQQFRKSKENNIFYSPISITSALGMVLLGAKDNTAQQISKVLHFDQVTENTTEKAATYHVDRSGNVHHQFQKLLTEFNKSTDAYELKIANKLFGEKTYQFLQEYLDAIKKFYQTSVESTDFANAPEESRKKINSWVESQTNEKIKNLFPDGTIGNDTTLVLVNAIYFKGQWENKFKKENTKEEKFWPNKNTYKSVQMMRQYNSFNFALLEDVQAKVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRETCVDLHLPRFKMEESYDLKDTLRTMGMVNIFNGDADLSGMTWSHGLSVSKVLHKAFVEVTEEGVEAAAATAVVVVELSSPSTNEEFCCNHPFLFFIRQNKTNSILFYGRFSSP Predict reactive species Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 4 Calculated Molecular Weight: 390 aa, 45 kDa Observed Molecular Weight: 42-45 kDa GenBank Accession Number: BC017401 Gene Symbol: SERPINB4 Gene ID (NCBI): 6318 RRID: AB_3085373 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P48594 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.