Proteintech, 11431-2-AP, SESN3 Polyclonal antibody

Size: 20ul / 150ul The SESN3 (11431-2-AP) by Proteintech is a Polyclonal antibody targeting SESN3 in WB, ELISA applications with reactivity to human, mouse, rat samples 11431-2-AP targets SESN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse ovary tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1961 Product name: Recombinant human SESN3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-321 aa of BC017296 Sequence: MNRGGGSPSAAANYLLCTNCRKVLRKDKRIRVSQPLTRGPSAFIPEKEVVQANTVDERTNFLVEEYSTSGRLDNITQVMSLHTQYLESFLRSQFYMLRMDGPLPLPYRHYIAIMAAARHQCSYLINMHVDEFLKTGGIAEWLNGLEYVPQRLKNLNEINKLLAHRPWLITKEHIQKLVKTGENNWSLPELVHAVVLLAHYHALASFVFGSGINPERDPEISNGFRLISVNNFCVCDLANDNNIENASLSGSNFGIVDSLSELEALMERMKRLQEEREDEEASQEEMSTRFEKEKKESLFVVSGDTFHSFPHSGAFLHFFAF Predict reactive species Full Name: sestrin 3 Calculated Molecular Weight: 492 aa, 57 kDa Observed Molecular Weight: 36 kDa, 51-59 kDa GenBank Accession Number: BC017296 Gene Symbol: SESN3 Gene ID (NCBI): 143686 RRID: AB_2185602 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P58005 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.