Proteintech, 11442-1-AP, PNPLA3 Polyclonal antibody

Size: 20ul / 150ul The PNPLA3 (11442-1-AP) by Proteintech is a Polyclonal antibody targeting PNPLA3 in WB, IHC, ELISA applications with reactivity to human, mouse samples 11442-1-AP targets PNPLA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: L02 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1959 Product name: Recombinant human PNPLA3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 79-181 aa of BC014449 Sequence: MPDDVLWLQWVTSQVFTRVLMCLLPASRSQMPVSSQQASPCTPEQDWPCWTPCSPEGCPAETKAEATPRSILRSSLNFFLGNKVPAGAEGLSTFPSFSLEKSL Predict reactive species Full Name: patatin-like phospholipase domain containing 3 Calculated Molecular Weight: 481 aa, 53 kDa Observed Molecular Weight: 50-70 kDa, 130-150 kDa GenBank Accession Number: BC014449 Gene Symbol: PNPLA3 Gene ID (NCBI): 80339 RRID: AB_2918026 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NST1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.