Proteintech, 11507-1-AP, FNTB Polyclonal antibody

Size: 20ul / 150ul The FNTB (11507-1-AP) by Proteintech is a Polyclonal antibody targeting FNTB in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 11507-1-AP targets FNTB in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, A431 cells, mouse skin tissue Positive IP detected in: HeLa cells Positive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2061 Product name: Recombinant human FNTB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-356 aa of BC020232 Sequence: MASPSSFTYYCPPSSSPVWSEPLYSLRPEHARERLQDDSVETVTSIEQAKVEEKIQEVFSSYKFNHLVPRLVLQREKHFHYLKRGLRQLTDAYECLDASRPWLCYWILHSLELLDEPIPQIVATDVCQFLELCQSPEGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYDIINREKLLQYLYSLKQPDGSFLMHVGGEVDVRSAYCAASVASLTNIITPDLFEGTAEWIARCQNWEGGIGGVPGMEAHGGYTFCGLAALVILKRERSLNLKSLLQWVTSRQMRFEGGFQGRCNKLVDGCYSFWQAGLLPLLHRALHAQGDPALSMSHWMFHQQALQEYILMCCQCPAGGLLDKPGK Predict reactive species Full Name: farnesyltransferase, CAAX box, beta Calculated Molecular Weight: 437 aa, 49 kDa Observed Molecular Weight: 49 kDa, 44 kDa GenBank Accession Number: BC020232 Gene Symbol: FNTB Gene ID (NCBI): 2342 RRID: AB_2106301 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P49356 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.