Proteintech, 11521-1-AP, ECM1 Polyclonal antibody

Size: 20ul / 150ul The ECM1 (11521-1-AP) by Proteintech is a Polyclonal antibody targeting ECM1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 11521-1-AP targets ECM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, L02 cells, HepG2 cells, mouse liver tissue, mouse kidney tissue, MCF-7 cells, mouse pancreas tissue, rat pancreas tissue, A375 cells Positive IHC detected in: human thyroid cancer tissue, human breast cancer tissue, human lung cancer tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis.ECM1 is expressed in breast cancer and could participate in cell migration and angiogenesis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(PMID:21128013) It inhibits MMP9 proteolytic activity. ECM1 protein is a possible trigger for angiogenesis, tumor progression and malignancies(PMID:21497598). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, canine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2084 Product name: Recombinant human ECM1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 241-540 aa of BC023505 Sequence: MSRFCEAEFSVKTRPHWCCTRQGEARFSCFQEEAPQPHYQLRACPSHQPDISSGLELPFPPGVPTLDNIKNICHLRRFRSVPRNLPATDPLQRELLALIQLEREFQRCCRQGNNHTCTWKAWEDTLDKYCDREYAVKTHHHLCCRHPPSPTRDECFARRAPYPNYDRDILTIDISRVTPNLMGHLCGNQRVLTKHKHIPGLIHNMTARCCDLPFPEQACCAEEEKLTFINDLCGPRRNIWRDPALCCYLSPGDEQVNCFNINYLRNVALVSGDTENAKGQGEQGSTGGTNISSTSEPKEE Predict reactive species Full Name: extracellular matrix protein 1 Calculated Molecular Weight: 540 aa, 61 kDa Observed Molecular Weight: 55-61 kDa GenBank Accession Number: BC023505 Gene Symbol: ECM1 Gene ID (NCBI): 1893 RRID: AB_2261964 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q16610 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.