Proteintech, 11573-1-AP, PSMA6 Polyclonal antibody

Size: 20ul / 150ul The PSMA6 (11573-1-AP) by Proteintech is a Polyclonal antibody targeting PSMA6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 11573-1-AP targets PSMA6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells, Neuro-2a cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human thyroid cancer tissue, human lung cancer tissue, human testis tissue, mouse brain tissue, mouse colon tissue, mouse testis tissue, rat small intestine tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, MCF-7 cells, HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information PSMA6(Proteasome subunit alpha type-6) is also named as PROS27 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It also has an ATP-dependent proteolytic activity. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, oyster Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2154 Product name: Recombinant human PSMA6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-246 aa of BC023659 Sequence: MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKLLDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLEKKVKKKFDWTFEQTVETAITCLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLVALAERD Predict reactive species Full Name: proteasome (prosome, macropain) subunit, alpha type, 6 Calculated Molecular Weight: 246 aa, 27 kDa Observed Molecular Weight: 27-29 kDa GenBank Accession Number: BC023659 Gene Symbol: PSMA6 Gene ID (NCBI): 5687 RRID: AB_2171742 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P60900 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.