Proteintech, 11596-1-AP, TFIIE Beta Polyclonal antibody

Size: 20ul / 150ul The TFIIE Beta (11596-1-AP) by Proteintech is a Polyclonal antibody targeting TFIIE Beta in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 11596-1-AP targets TFIIE Beta in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, A375 cells, Jurkat cells Positive IF/ICC detected in: A375 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex, which plays pivotal roles in initiation of protein-coding gene transcription (PMID: 7829083). Transcription initiation factor IIE comprises two subunits, TFIIE-alpha and TFIIE-beta, two chains of which together form a tetramer. TFIIE-beta is encoded by GTF2E2 gene and contains TFIIE beta-DNA binding domain. It mainly functions to recruit TFIIH to the initiation complex and stimulate RNA polymerase II kinase activity (PMID: 10792464). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2136 Product name: Recombinant human TFIIE beta protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 3-291 aa of BC030572 Sequence: PSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNGSFNLKALSGSSGYKFGVLAKIVNYMKTRHQRGDTHPLTLDEILDETQHLDIGLKQKQWLMTEALVNNPKIEVIDGKYAFKPKYNVRDKKALLRLLDQHDQRGLGGILLEDIEEALPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQFSVDEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGVLKDYSDITSSK Predict reactive species Full Name: general transcription factor IIE, polypeptide 2, beta 34kDa Calculated Molecular Weight: 291 aa, 33 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC030572 Gene Symbol: GTF2E2 Gene ID (NCBI): 2961 RRID: AB_2232722 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P29084 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.