Proteintech, 11604-1-AP, ALDH4A1 Polyclonal antibody

Size: 20ul / 150ul The ALDH4A1 (11604-1-AP) by Proteintech is a Polyclonal antibody targeting ALDH4A1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 11604-1-AP targets ALDH4A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human heart tissue, HepG2 cells, human brain tissue, human liver tissue, human skeletal muscle tissue, K-562 cells, mouse skeletal muscle tissue, mouse small intestine tissue, rat heart tissue Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: human liver cancer tissue, human liver tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ALDH4A1(Aldehyde dehydrogenase family 4 member A1) is also named as ALDH4, P5CDH,P5C dehydrogenase and belongs to the aldehyde dehydrogenase family. P5CDH is a mitochondrial matrix NAD(+)-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate.Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2147 Product name: Recombinant human ALDH4A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 246-563 aa of BC023600 Sequence: YRILREAGLPPNIIQFVPADGPLFGDTVTSSEHLCGINFTGSVPTFKHLWKQVAQNLDRFHTFPRLAGECGGKNFHFVHRSADVESVVSGTLRSAFEYGGQKCSACSRLYVPHSLWPQIKGRLLEEHSRIKVGDPAEDFGTFFSAVIDAKSFARIKKWLEHARSSPSLTILAGGKCDDSVGYFVEPCIVESKDPQEPIMKEEIFGPVLSVYVYPDDKYKETLQLVDSTTSYGLTGAVFSQDKDVVQEATKVLRNAAGNFYINDKSTGSIVGQQPFGGARASGTNDKPGGPHYILRWTSPQVIKETHKPLGDWSYAYMQ Predict reactive species Full Name: aldehyde dehydrogenase 4 family, member A1 Calculated Molecular Weight: 563 aa, 62 kDa Observed Molecular Weight: 62 kDa GenBank Accession Number: BC023600 Gene Symbol: ALDH4A1 Gene ID (NCBI): 8659 RRID: AB_2258046 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P30038 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.