Proteintech, 11621-1-AP, NEIL3 Polyclonal antibody

Size: 20ul / 150ul The NEIL3 (11621-1-AP) by Proteintech is a Polyclonal antibody targeting NEIL3 in WB, ELISA applications with reactivity to human, mouse samples 11621-1-AP targets NEIL3 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, A431 cells, mouse skin tissue, human skin tissue, THP-1 cells, U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389). Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2200 Product name: Recombinant human NEIL3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 257-605 aa of BC025954 Sequence: CHCRITVCRFGDNNRMTYFCPHCQKENPQHVDICKLPTRNTIISWTSSRVDHVMDSVARKSEEHWTCVVCTLINKPSSKACDACLTSRPIDSVLKSEENSTVFSHLMKYPCNTFGKPHTEVKINRKTAFGTTTLVLTDFSNKSSTLERKTKQNQILDEEFQNSPPASVCLNDIQHPSKKTTNDITQLSSKVNISPTISSESKLFSPAHKKPKTAHYSSPELKSCNPGYSNSELQINMTDGPRTLNPDSPRCSKHNRLCILRVVRKDGENKGRQFYACPLPREAQCGFFEWADLSFPFCNHGKRSTMKTVLKIGPNNGKNFFVCPLGKEKQCNFFQWAENGPGIKIIPGC Predict reactive species Full Name: nei endonuclease VIII-like 3 (E. coli) Calculated Molecular Weight: 605 aa, 68 kDa Observed Molecular Weight: 62-68 kDa GenBank Accession Number: BC025954 Gene Symbol: NEIL3 Gene ID (NCBI): 55247 RRID: AB_2251229 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TAT5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.