Proteintech, 11642-1-AP, SAFB2 Polyclonal antibody

Size: 20ul / 150ul The SAFB2 (11642-1-AP) by Proteintech is a Polyclonal antibody targeting SAFB2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, rat samples 11642-1-AP targets SAFB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, BxPC-3 cells, HeLa cells, rat pancreas tissue Positive IHC detected in: human breast cancer tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation. Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2230 Product name: Recombinant human SAFB2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC025279 Sequence: MAETLPGSGDSGPGTASLGPGVAETGTRRLSELRVIDLRAELKKRNLDTGGNKSVLMERLKKAVKEEGQDPDEIGIELEATSKKSAKRCVKGLKMEEEGTEDNGLEDDSRDGQEDMEASLENLQNMGMMDMSVLDETEVANSSAPDFGEDGTDGLLDSFCDSKEYVAAQLRQLPAQPPEHAVDGEGFKNTLETSSLNFKVTPDIEESLLEPENEKILDILGETCKSEPVKEESSELEQPFAQDTSSVGPDRKLAEEEDLFDSAHPEEGDLDLASESTAHAQSSKADSLLAVVKREPAEQPGDGERTDCEPVGLEPAVEQSSAASELAEASSEELAEAPTEAPSPEARDS Predict reactive species Full Name: scaffold attachment factor B2 Calculated Molecular Weight: 528aa,57 kDa; 953aa,107 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: BC025279 Gene Symbol: SAFB2 Gene ID (NCBI): 9667 RRID: AB_2301309 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14151 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.