Proteintech, 11753-1-AP, TMOD4 Polyclonal antibody

Size: 20ul / 150ul The TMOD4 (11753-1-AP) by Proteintech is a Polyclonal antibody targeting TMOD4 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 11753-1-AP targets TMOD4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse skeletal muscle tissue, HeLa cells, human brain tissue Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: mouse skeletal muscle tissue, human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TMOD4 is a member of tropomodulin (Tmod) family proteins which are capping proteins binding to the pointed end of actin filaments and preventing both elongation and depolymerization. Four mammalian Tmod genes have been identified to date. Tomd1 is expressed in terminally-differentiated post-mitotic cells, like heart, brain, lens, vasculature, and erythrocytes; Tmod2 expressed in neurons; Tmod3 expressed ubiquitously; Tmod4 expressed only in skeletal muscles. This antibody was raised against the full-length human TMOD4 and may cross-react with other Tmods. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse, rat, zebrafish, xenopus, branchiostoma belcheri Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2349 Product name: Recombinant human TMOD4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC017810 Sequence: MSSYQKELEKYRDIDEDEILRTLSPEELEQLDCELQEMDPENMLLPAGLRQRDQTKKSPTGPLDREALLQYLEQQALEVKERDDLVPFTGEKKGKPYIQPKREIPAEEQITLEPELEEALAHATDAEMCDIAAILDMYTLMSNKQYYDALCSGEICNTEGISSVVQPDKYKPVPDEPPNPTNIEEILKRVRSNDKELEEVNLNNIQDIPIPMLSELCEAMKANTYVRSFSLVATRSGDPIANAVADMLRENRSLQSLNIESNFISSTGLMAVLKAVRENATLTELRVDNQRQWPGDAVEMEMATVLEQRPSIVRFGYHFTQQGPRARAAQAMTRNNELRRQQKKR Predict reactive species Full Name: tropomodulin 4 (muscle) Calculated Molecular Weight: 345 aa, 39 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC017810 Gene Symbol: TMOD4 Gene ID (NCBI): 29765 RRID: AB_2205433 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NZQ9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.